Human MIP-1 Alpha (Macrophage Inflammatory Protein 1 Alpha) Sandwich ELISA Kit (STJE0019880)

Name: Human MIP-1 Alpha (Macrophage Inflammatory Protein 1 Alpha) Sandwich ELISA Kit (STJE0019880)
SKU: STJE0019880
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SPECIFICATIONS

STJE0019880

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Short Description	This MIP-1 Alpha Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	1.62 pg/mL
Detection Limit	3.13-200 pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	CCL3
Gene ID	6348
Uniprot ID	CCL3_HUMAN
Specificity	This kit recognizes Human MIP-1 Alpha in samples. No significant cross-reactivity or interference between Human MIP-1 Alpha and analogues was observed.
Sample Type	serum, plasma and other biological fluids

Post Translational Modifications	N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
Function	Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Protein Name	C-C Motif Chemokine 3 G0/G1 Switch Regulatory Protein 19-1 Macrophage Inflammatory Protein 1-Alpha Mip-1-Alpha Pat 464.1 Sis-Beta Small-Inducible Cytokine A3 Tonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69 Ld78-Alpha(4-69
Database Links	Reactome: R-HSA-380108 Reactome: R-HSA-6783783
Cellular Localisation	Secreted
Alternative ELISA Names	C-C Motif Chemokine 3 ELISA kit G0/G1 Switch Regulatory Protein 19-1 ELISA kit Macrophage Inflammatory Protein 1-Alpha ELISA kit Mip-1-Alpha ELISA kit Pat 464.1 ELISA kit Sis-Beta ELISA kit Small-Inducible Cytokine A3 ELISA kit Tonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69 ELISA kit Ld78-Alpha(4-69 ELISA kit CCL3 ELISA kit G0S19-1 ELISA kit MIP1A ELISA kit SCYA3 ELISA kit
Specificity	This kit recognizes Human MIP-1α in samples. No significant cross-reactivity or interference between Human MIP-1α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	10.25	20.84	78.04	10.29	22.66	72.89
Standard deviation	0.53	1.03	2.81	0.65	1.21	3.00
CV (%)	5.18	4.94	3.60	6.36	5.36	4.11