Human MIF (MIF) Antibody Pair (STJAP00733)
General Information
| Short Description | This MIF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R02-1N7/R07-1X2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MIF |
| Gene ID | 4282 |
| Uniprot ID | MIF_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Function | Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. |
| Protein Name | Macrophage Migration Inhibitory FactorMifGlycosylation-Inhibiting FactorGifL-Dopachrome IsomeraseL-Dopachrome TautomerasePhenylpyruvate Tautomerase |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-6798695Reactome: R-HSA-8950505 |
| Cellular Localisation | SecretedCytoplasmDoes Not Have A Cleavable Signal Sequence And Is Secreted Via A SpecializedNon-Classical PathwaySecreted By Macrophages Upon Stimulation By Bacterial Lipopolysaccharide (Lps)Or By MTuberculosis Antigens |
| Alternative Antibody Pair Names | Macrophage Migration Inhibitory Factor antibody pairMif antibody pairGlycosylation-Inhibiting Factor antibody pairGif antibody pairL-Dopachrome Isomerase antibody pairL-Dopachrome Tautomerase antibody pairPhenylpyruvate Tautomerase antibody pairMIF antibody pairGLIF antibody pairMMIF antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human MIF (MIF) Antibody Pair (STJAP00733)
This antibody pair is designed to detect human Macrophage Migration Inhibitory Factor (MIF), corresponding to UniProt entry P14174. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. Clone R02-1N7 and clone R07-1X2 are both affinity purified.
This antibody pair is validated for use in ELISA. No other assay formats are specified for this product.
Optimal working concentrations are to be determined by the end user. Titration experiments are recommended to establish the appropriate dilution for each specific experimental setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Both rabbit monoclonal antibodies were raised against a recombinant protein immunogen and purified by affinity chromatography. No immunogen region is specified in the product details.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.