Human MIF (Macrophage Migration Inhibitory Factor) Antibody Pair (STJAP00460)
General Information
| Short Description | This MIF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | MIF |
| Gene ID | 4282 |
| Uniprot ID | MIF_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human MIF protien.Detection Immunogen: Recombinant Human MIF protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Protein Name | Macrophage Migration Inhibitory FactorMifGlycosylation-Inhibiting FactorGifL-Dopachrome IsomeraseL-Dopachrome TautomerasePhenylpyruvate Tautomerase |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-6798695Reactome: R-HSA-8950505 |
| Cellular Localisation | SecretedCytoplasmDoes Not Have A Cleavable Signal Sequence And Is Secreted Via A SpecializedNon-Classical PathwaySecreted By Macrophages Upon Stimulation By Bacterial Lipopolysaccharide (Lps)Or By MTuberculosis Antigens |
| Alternative Antibody Pair Names | Macrophage Migration Inhibitory Factor antibody pairMif antibody pairGlycosylation-Inhibiting Factor antibody pairGif antibody pairL-Dopachrome Isomerase antibody pairL-Dopachrome Tautomerase antibody pairPhenylpyruvate Tautomerase antibody pairMIF antibody pairGLIF antibody pairMMIF antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human MIF (Macrophage Migration Inhibitory Factor) Antibody Pair (STJAP00460)
This antibody pair detects Human Macrophage Migration Inhibitory Factor (MIF), corresponding to UniProt entry P14174. Reactivity is confirmed for human samples only.
The capture antibody is unconjugated and the detection antibody is biotin-conjugated. Both are supplied at 0.5 mg/mL, are mouse-derived, and were purified by Protein A or G.
This pair is validated for use in ELISA. It has not been described as validated for other assay formats such as lateral flow or multiplex.
Compatible sample types include serum, plasma, and other biological fluids. This product is for research use only and is not intended for diagnostic or therapeutic applications.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Both the capture and detection antibodies were raised against recombinant Human MIF protein. No further information regarding the specific immunogen region is provided in the product details.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.