Human MICA protein (Recombinant-Active) (C-His) (STJP012129)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human MICA-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Immobilised recombinant human MICA at 2 Mu g/mL (100 Mu L/well) can bind NKG2D with a linear range of 0.61-14.3 ng/mL. |
Target Information
| Gene Symbol | MICA |
| Gene ID | 100507436 |
| Uniprot ID | MICA_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Gln308 |
Additional Info
| Post Translational Modifications | N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. Palmitoylated on cysteine residues in the cytoplasmic tail leading to its association with membrane microdomains enriched in cholesterol. N-glycosylation is necessary for cell surface expression. (Microbial infection) Ubiquitinated by human herpesvirus 8 protein K5, leading to degradation. |
| Function | Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production. |
| Protein Name | Mhc Class I Polypeptide-Related Sequence AMic-A |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmExpressed On The Cell Surface In Gastric EpitheliumEndothelial Cells And Fibroblasts And In The Cytoplasm In Keratinocytes And MonocytesInfection With Human Adenovirus 5 Suppresses Cell Surface Expression Due To The Adenoviral E3-19k Protein Which Causes Retention In The Endoplasmic Reticulum |
| Alternative Protein Names | Mhc Class I Polypeptide-Related Sequence A proteinMic-A proteinMICA proteinPERB11.1 protein |
Information sourced from Uniprot.org