Human MGMT protein (Recombinant) (His-Tag) (STJP019705)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human MGMT-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Target Information
| Gene Symbol | MGMT |
| Gene ID | 4255 |
| Uniprot ID | MGMT_HUMAN |
| Accession Number | NP_002403.3 |
| Immunogen | Human |
| Immunogen Region | 1-207aa |
| Immunogen Sequence |
Additional Info
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Protein Name | Methylated-Dna--Protein-Cysteine Methyltransferase6-O-Methylguanine-Dna MethyltransferaseMgmtO-6-Methylguanine-Dna-Alkyltransferase |
| Database Links | Reactome: R-HSA-5657655 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Methylated-Dna--Protein-Cysteine Methyltransferase protein6-O-Methylguanine-Dna Methyltransferase proteinMgmt proteinO-6-Methylguanine-Dna-Alkyltransferase proteinMGMT protein |
Information sourced from Uniprot.org