Human MEP1B protein (Recombinant-Active) (C-10*His) (STJP012126)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human MEP1B-C-10*His protein was developed from mammalian cells and has a target region of C-10*His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to cleave a fluorogenic peptide substrate, McaSEVNLDAEFRK (Dpn) RRNH2. The specific activity is >350 pmol/min/µg, as measured under the described conditions. |
Target Information
| Gene Symbol | MEP1B |
| Gene ID | 4225 |
| Uniprot ID | MEP1B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Leu21-Ser593 |
Additional Info
| Post Translational Modifications | Phosphorylated by PKC at multiple sites of its cytoplasmic part. Phosphorylation dcreases activity at the cell surface, leading to diminished substrate cleavage. N-glycosylated.contains high mannose and/or complex biantennary structures. O-glycosylation protect the C-terminal region from proteolytic cleavage and diminish secretion, this seems to be specific to human. Proteolytically activated by trypsin in the intestinal lumen and kallikrein-related peptidases in other tissues. |
| Function | Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. Also cleaves the amyloid precursor protein/APP, thereby releasing neurotoxic amyloid beta peptides. |
| Protein Name | Meprin A Subunit BetaEndopeptidase-2Meprin BN-Benzoyl-L-Tyrosyl-P-Amino-Benzoic Acid Hydrolase Subunit BetaPaba Peptide HydrolasePph Beta |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSecretedHomodimers Are Essentially Membrane Bound But May Also Be Shed From The Surface By Adam-10 And Adam-17 |
| Alternative Protein Names | Meprin A Subunit Beta proteinEndopeptidase-2 proteinMeprin B proteinN-Benzoyl-L-Tyrosyl-P-Amino-Benzoic Acid Hydrolase Subunit Beta proteinPaba Peptide Hydrolase proteinPph Beta proteinMEP1B protein |
Information sourced from Uniprot.org