Human MCP-1 (MCP-1) Antibody Pair (STJAP00675)
General Information
| Short Description | This MCP-1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 4A2/6E1 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CCL2 |
| Gene ID | 6347 |
| Uniprot ID | CCL2_HUMAN |
| Immunogen | A synthesized peptide derived from CCL2 |
Additional Info
| Tissue Specificity | Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes. |
| Post Translational Modifications | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. N-Glycosylated. |
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Protein Name | C-C Motif Chemokine 2Hc11Monocyte Chemoattractant Protein 1Monocyte Chemotactic And Activating FactorMcafMonocyte Chemotactic Protein 1Mcp-1Monocyte Secretory Protein JeSmall-Inducible Cytokine A2 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9818026 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | C-C Motif Chemokine 2 antibody pairHc11 antibody pairMonocyte Chemoattractant Protein 1 antibody pairMonocyte Chemotactic And Activating Factor antibody pairMcaf antibody pairMonocyte Chemotactic Protein 1 antibody pairMcp-1 antibody pairMonocyte Secretory Protein Je antibody pairSmall-Inducible Cytokine A2 antibody pairCCL2 antibody pairMCP1 antibody pairSCYA2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human MCP-1 (MCP-1) Antibody Pair (STJAP00675)
This antibody pair detects human MCP-1 (CCL2, UniProt P13500). Reactivity is confirmed for human samples only.
The pair consists of two mouse monoclonal antibodies with clone IDs 4A2 and 6E1, both of IgG2b isotype. Both antibodies are supplied unconjugated, affinity purified, at 1 mg/mL in liquid format.
This antibody pair has been validated for use in ELISA at a dilution of 1:1000. No other assay formats are documented for this product.
One antibody is formulated in PBS containing 0.09% sodium azide, and the other is formulated in PBS containing 0.01% thimerosal. Researchers should take appropriate precautions when handling these preservatives, particularly when using enzyme-based detection systems sensitive to azide.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Both antibodies were raised against a synthesized peptide derived from human CCL2 (MCP-1). This product is for research use only and is not intended for diagnostic or therapeutic applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.