Human MCP-1 (C-C Motif Chemokine 2) Antibody Pair (STJAP00046)
General Information
| Short Description | This MCP-1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CCL2 |
| Gene ID | 6347 |
| Uniprot ID | CCL2_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human MCP-1 protein.Detection Immunogen: Recombinant Human MCP-1 protein. |
Additional Info
| Post Translational Modifications | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. N-Glycosylated. |
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Protein Name | C-C Motif Chemokine 2Hc11Monocyte Chemoattractant Protein 1Monocyte Chemotactic And Activating FactorMcafMonocyte Chemotactic Protein 1Mcp-1Monocyte Secretory Protein JeSmall-Inducible Cytokine A2 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9818026 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | C-C Motif Chemokine 2 antibody pairHc11 antibody pairMonocyte Chemoattractant Protein 1 antibody pairMonocyte Chemotactic And Activating Factor antibody pairMcaf antibody pairMonocyte Chemotactic Protein 1 antibody pairMcp-1 antibody pairMonocyte Secretory Protein Je antibody pairSmall-Inducible Cytokine A2 antibody pairCCL2 antibody pairMCP1 antibody pairSCYA2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human MCP-1 (C-C Motif Chemokine 2) Antibody Pair (STJAP00046)
This antibody pair detects Human MCP-1 (C-C Motif Chemokine 2, UniProt P13500) and is validated for reactivity against human samples only. Both the capture and detection antibodies were raised against recombinant Human MCP-1 protein.
The capture antibody is a mouse monoclonal antibody supplied unconjugated, while the detection antibody is a goat polyclonal antibody supplied with a biotin conjugate. Both components are provided at a concentration of 0.5 mg/mL.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
The capture antibody was purified by Protein A or G affinity chromatography, and the detection antibody was purified by antigen affinity chromatography. Both antibodies are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.