Human MCL1 protein (Recombinant) (His-Tag) (STJP017805)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human MCL1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% Glycerol, 2mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCL1 |
| Gene ID | 4170 |
| Uniprot ID | MCL1_HUMAN |
| Accession Number | NP_068779.1 |
| Immunogen | Human |
| Immunogen Region | 1-327aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159. Deubiquitinated by USP20.leading to increased stability. |
| Function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
| Protein Name | Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1Bcl-2-Like Protein 3Bcl2-L-3Bcl-2-Related Protein Eat/Mcl1Mcl1/Eat |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9725370 |
| Cellular Localisation | MembraneSingle-Pass Membrane ProteinCytoplasmMitochondrionNucleusNucleoplasmCytoplasmicAssociated With Mitochondria |
| Alternative Protein Names | Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 proteinBcl-2-Like Protein 3 proteinBcl2-L-3 proteinBcl-2-Related Protein Eat/Mcl1 proteinMcl1/Eat proteinMCL1 proteinBCL2L3 protein |
Information sourced from Uniprot.org