Human MBP-C/MBL2 (Mannose-binding protein C) Sandwich ELISA Kit (STJE0002870)

Name: Human MBP-C/MBL2 (Mannose-binding protein C) Sandwich ELISA Kit (STJE0002870)
SKU: STJE0002870
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SPECIFICATIONS

STJE0002870

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Short Description	This MBP-C/MBL2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	MBL2
Gene ID	4153
Uniprot ID	MBL2_HUMAN
Specificity	This kit recognizes Human MBP-C/MBL2 in samples. No significant cross-reactivity or interference between Human MBP-C/MBL2 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response.
Protein Name	Mannose-Binding Protein C Mbp-C Collectin-1 Mbp1 Mannan-Binding Protein Mannose-Binding Lectin
Database Links	Reactome: R-HSA-166662 Reactome: R-HSA-166663 Reactome: R-HSA-9705671
Cellular Localisation	Secreted
Alternative ELISA Names	Mannose-Binding Protein C ELISA kit Mbp-C ELISA kit Collectin-1 ELISA kit Mbp1 ELISA kit Mannan-Binding Protein ELISA kit Mannose-Binding Lectin ELISA kit MBL2 ELISA kit COLEC1 ELISA kit MBL ELISA kit
Specificity	This kit recognizes Human MBP-C/MBL2 in samples. No significant cross-reactivity or interference between Human MBP-C/MBL2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.52	1.58	4.58	0.52	1.59	5.01
Standard deviation	0.03	0.07	0.23	0.03	0.07	0.17
CV (%)	5.77	4.43	5.02	5.77	4.40	3.39