Human MB (Myoglobin) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human MB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 0.38ng/mL |
| Detection Limit | 0.63~40ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | MB |
| Gene ID | 4151 |
| Uniprot ID | MYG_HUMAN |
| Specificity | This kit recognizes Human MYO in samples. No significant cross-reactivity or interference between Human MYO and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand. Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity. |
| Protein Name | MyoglobinNitrite Reductase MbPseudoperoxidase Mb |
| Database Links | Reactome: R-HSA-8981607 |
| Cellular Localisation | CytoplasmSarcoplasm |
| Alternative CLIA Names | Myoglobin CLIA kitNitrite Reductase Mb CLIA kitPseudoperoxidase Mb CLIA kitMB CLIA kit |
| Specificity | This kit recognizes Human MYO in samples. No significant cross-reactivity or interference between Human MYO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma(n=8) | 88-103 | 96 |
| Cell culture media(n=8) | 100-113 | 107 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.22 | 5.24 | 18.71 | 2.20 | 5.39 | 20.37 |
| Standard deviation | 0.26 | 0.61 | 1.39 | 0.27 | 0.54 | 2.23 |
| CV (%) | 11.71 | 11.64 | 7.43 | 12.27 | 10.02 | 10.95 |