Human Mature TGF-beta 2 protein (Recombinant) (No tag) (STJP002625)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human TGF-beta 2 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Ala303-Ser414) of human TGF-beta 2/TGFB2 (Accession #P61812).
General Information
| Short Description | Recombinant-Human Mature TGF-beta 2-No tag protein was developed in hek293 cells using the region Ala303-Ser414. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.2 Mu m filtered solution of 4 mM HCl. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Recombinant Human Mature TGF-beta 2 Protein inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.058-0.232 ng/mL, corresponding to a specific activity of 4.3×106~1.72×107 units/mg. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | TGFB2 |
| Gene ID | 7042 |
| Uniprot ID | TGFB2_HUMAN |
| Immunogen | Recombinant Human TGF-beta 2 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Ala303-Ser414) of human TGF-beta 2/TGFB2 (Accession #P61812). |
| Immunogen Region | Ala303-Ser414 |
| Immunogen Sequence | ALDAAYCFRNVQDNCCLRPL YIDFKRDLGWKWIHEPKGYN ANFCAGACPYLWSSDTQHSR VLSLYNTINPEASASPCCVS QDLEPLTILYYIGKTPKIEQ LSNMIVKSCKCS |
Additional Info
| Function | Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. |
| Protein Name | Transforming Growth Factor Beta-2 ProproteinCeterminGlioblastoma-Derived T-Cell Suppressor FactorG-Tsf Cleaved Into - Latency-Associated PeptideLap - Transforming Growth Factor Beta-2Tgf-Beta-2 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-2129379Reactome: R-HSA-2173789Reactome: R-HSA-3000178Reactome: R-HSA-9839389 |
| Cellular Localisation | Latency-Associated Peptide: SecretedExtracellular SpaceExtracellular MatrixTransforming Growth Factor Beta-2: Secreted |
| Alternative Protein Names | Transforming Growth Factor Beta-2 ProproteinCetermin proteinGlioblastoma-Derived T-Cell Suppressor Factor proteinG-Tsf Cleaved Into - Latency-Associated Peptide proteinLap - Transforming Growth Factor Beta-2 proteinTgf-Beta-2 proteinTGFB2 protein |
Information sourced from Uniprot.org