Human Mature GDNF protein (Recombinant) (NO-Tag) (STJP020065)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human GDNF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser78-Ile211) of human GDNF (Accession #P39905).
General Information
| Short Description | Recombinant-Human Mature GDNF-NO-Tag protein was developed in e.coli using the region Ser78-Ile211. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method |
Target Information
| Gene Symbol | GDNF |
| Gene ID | 2668 |
| Uniprot ID | GDNF_HUMAN |
| Immunogen | Recombinant Human GDNF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser78-Ile211) of human GDNF (Accession #P39905). |
| Immunogen Region | Ser78-Ile211 |
| Immunogen Sequence | SPDKQMAVLPRRERNRQAAA ANPENSRGKGRRGQRGKNRG CVLTAIHLNVTDLGLGYETK EELIFRYCSGSCDAAETTYD KILKNLSRNRRLVSDKVGQA CCRPIAFDDDLSFLDDNLVY HILRKHSAKRCGCI |
Additional Info
| Tissue Specificity | In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. |
| Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor. Involved in the development of the neural crest. |
| Protein Name | Glial Cell Line-Derived Neurotrophic FactorHgdnfAstrocyte-Derived Trophic FactorAtf |
| Database Links | Reactome: R-HSA-419037Reactome: R-HSA-5673001Reactome: R-HSA-8853659Reactome: R-HSA-9830674 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Glial Cell Line-Derived Neurotrophic Factor proteinHgdnf proteinAstrocyte-Derived Trophic Factor proteinAtf proteinGDNF protein |
Information sourced from Uniprot.org