Human Mature BMP-7 protein (Recombinant) (N-His＆N-hFC) (STJP003029)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human BMP-7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser293-His431) of Human BMP-7 (Accession #NP_001710.1) fused with His tag at the N-terminus
General Information
| Short Description | Recombinant-Human Mature BMP-7-N-His＆N-hFC protein was developed in hek293 cells using the region Ser293-His431. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 50mM acetic acid, pH 3.6. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | BMP7 |
| Gene ID | 655 |
| Uniprot ID | BMP7_HUMAN |
| Immunogen | Recombinant Human BMP-7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser293-His431) of Human BMP-7 (Accession #NP_001710.1) fused with His tag at the N-terminus |
| Immunogen Region | Ser293-His431 |
| Immunogen Sequence | RAQMDPNRISEDSTHCFYRI LRLHENADLQDSTLESEDTL PDSCRRMKQAFQGAVQKELQ HIVGPQRFSGAPAMMEGSWL DVAQRGKPEAQPFAHLTINA ASIPSGSHKVTLSSWYHDRG WAKISNMTLSNGKLRVNQDG FYYLYANICFRHHETSGSVP TDYLQLMVYVVKTSIKIPSS HNLMKGGSTKNWSGNSEFHF YSINVGGFFKLRAGEEISIQ VSNPSLLDPDQDATYFGAF |
Additional Info
| Tissue Specificity | Expressed in the kidney and bladder. Lower levels seen in the brain. |
| Post Translational Modifications | Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms. |
| Function | Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A. Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, also uses BMPR2 as type II receptor. Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors. Promotes the expression of HAMP, this is repressed by its interaction with ERFE. |
| Protein Name | Bone Morphogenetic Protein 7Bmp-7Osteogenic Protein 1Op-1Eptotermin Alfa |
| Database Links | Reactome: R-HSA-2129379Reactome: R-HSA-9844594 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Bone Morphogenetic Protein 7 proteinBmp-7 proteinOsteogenic Protein 1 proteinOp-1 proteinEptotermin Alfa proteinBMP7 proteinOP1 protein |
Information sourced from Uniprot.org