Human MAP1LC3C protein (Recombinant) (N-His) (STJP007853)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human MAP1LC3C-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | MAP1LC3C |
| Gene ID | 440738 |
| Uniprot ID | MLP3C_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Arg11-Leu147 |
Additional Info
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-96 and Ser-98 by TBK1 prevents interaction with ATG4 (ATG4A, ATG4B, ATG4C or ATG4D). Phosphorylation by TBK1 on autophagosomes prevents their delipidation by ATG4 and premature removal from nascent autophagosomes. |
| Function | Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruits all ATG8 family members to infecting bacteria such as S.typhimurium. May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins. |
| Protein Name | Microtubule-Associated Protein 1 Light Chain 3 GammaAutophagy-Related Protein Lc3 CAutophagy-Related Ubiquitin-Like Modifier Lc3 CMap1 Light Chain 3-Like Protein 3Microtubule-Associated Proteins 1a/1b Light Chain 3cMap1a/Map1b Lc3 CMap1a/Map1b Light Chain 3 C |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosome MembraneLipid-AnchorEndomembrane SystemCytoplasmCytoskeletonLc3-Ii Binds To The Autophagic Membranes |
| Alternative Protein Names | Microtubule-Associated Protein 1 Light Chain 3 Gamma proteinAutophagy-Related Protein Lc3 C proteinAutophagy-Related Ubiquitin-Like Modifier Lc3 C proteinMap1 Light Chain 3-Like Protein 3 proteinMicrotubule-Associated Proteins 1a/1b Light Chain 3c proteinMap1a/Map1b Lc3 C proteinMap1a/Map1b Light Chain 3 C proteinMAP1LC3C protein |
Information sourced from Uniprot.org