Human MAGEA3 protein (Recombinant) (His-Tag) (STJP016897)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human MAGEA3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol, 100mM NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Target Information
| Gene Symbol | MAGEA3 |
| Gene ID | 4102 |
| Uniprot ID | MAGA3_HUMAN |
| Accession Number | NP_005353.1 |
| Immunogen | Human |
| Immunogen Region | 1-314aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas. |
| Post Translational Modifications | Ubiquitinated by the DCX(DCAF12) complex specifically recognizes the diglutamate (Glu-Glu) at the C-terminus, leading to its degradation. |
| Function | Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. |
| Protein Name | Melanoma-Associated Antigen 3Antigen Mz2-DCancer/Testis Antigen 1.3Ct1.3Mage-3 Antigen |
| Database Links | |
| Cellular Localisation | |
| Alternative Protein Names | Melanoma-Associated Antigen 3 proteinAntigen Mz2-D proteinCancer/Testis Antigen 1.3 proteinCt1.3 proteinMage-3 Antigen proteinMAGEA3 proteinMAGE3 protein |
Information sourced from Uniprot.org