Human MADCAM1 protein (Recombinant) (C-His) (STJP020357)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human MADCAM1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Gln317) of human MADCAM1 (Accession #NP_570116.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human MADCAM1-C-His protein was developed in hek293 cells using the region Gln19-Gln317. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MADCAM1 |
| Gene ID | 8174 |
| Uniprot ID | MADCA_HUMAN |
| Immunogen | Recombinant Human MADCAM1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Gln317) of human MADCAM1 (Accession #NP_570116.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Gln19-Gln317 |
| Immunogen Sequence | QSLQVKPLQVEPPEPVVAVA LGASRQLTCRLACADRGASV QWRGLDTSLGAVQSDTGRSV LTVRNASLSAAGTRVCVGSC GGRTFQHTVQLLVYAFPDQL TVSPAALVPGDPEVACTAHK VTPVDPNALSFSLLVGGQEL EGAQALGPEVQEEEEEPQGD EDVLFRVTERWRLPPLGTPV PPALYCQATMRLPGLELSHR QAIPVLHSPTSPEPPDTTSP ESPDTTSPESPDTTSQEPP |
Additional Info
| Tissue Specificity | Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. |
| Post Translational Modifications | The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. |
| Function | Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. |
| Protein Name | Mucosal Addressin Cell Adhesion Molecule 1Madcam-1Hmadcam-1 |
| Database Links | Reactome: R-HSA-198933 Q13477-1Reactome: R-HSA-216083 Q13477-1 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Mucosal Addressin Cell Adhesion Molecule 1 proteinMadcam-1 proteinHmadcam-1 proteinMADCAM1 protein |
Information sourced from Uniprot.org