Human LTA/TNF-beta protein (Recombinant) (His-Tag) (STJP016658)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human LTA/TNF-beta-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Bioactivity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 30% Glycerol, 0.1M NaCl, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range โค 1ng/ml. |
Target Information
| Gene Symbol | LTA |
| Gene ID | 4049 |
| Uniprot ID | TNFB_HUMAN |
| Accession Number | NP_001153212 |
| Immunogen | Human |
| Immunogen Region | 35-205aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL |
Additional Info
| Function | Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo. |
| Protein Name | Lymphotoxin-AlphaLt-AlphaTnf-BetaTumor Necrosis Factor Ligand Superfamily Member 1 |
| Database Links | Reactome: R-HSA-5668541Reactome: R-HSA-5669034Reactome: R-HSA-5676594 |
| Cellular Localisation | SecretedMembraneThe Homotrimer Is SecretedThe Heterotrimer Is Membrane-Associated |
| Alternative Protein Names | Lymphotoxin-Alpha proteinLt-Alpha proteinTnf-Beta proteinTumor Necrosis Factor Ligand Superfamily Member 1 proteinLTA proteinTNFB proteinTNFSF1 protein |
Information sourced from Uniprot.org