Human LpPLA2 (Platelet-Activating Factor Acetylhydrolase) Antibody Pair (STJAP00045)
General Information
| Short Description | This LpPLA2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PLA2G7 |
| Gene ID | 7941 |
| Uniprot ID | PAFA_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human LpPLA2 protein.Detection Immunogen: Recombinant Human LpPLA2 protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles. |
| Function | Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage. |
| Protein Name | Platelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase1-Alkyl-2-Acetylglycerophosphocholine Esterase2-Acetyl-1-Alkylglycerophosphocholine EsteraseGroup-Viia Phospholipase A2Gviia-Pla2Ldl-Associated Phospholipase A2Ldl-Pla(2Paf 2-Acylhydrolase |
| Database Links | Reactome: R-HSA-422085 |
| Cellular Localisation | SecretedExtracellular SpaceAssociates With Both Ldl And Hdl Particles In PlasmaMainly Associates With Pro-Inflammatory Electronegative Ldl Particles |
| Alternative Antibody Pair Names | Platelet-Activating Factor Acetylhydrolase antibody pairPaf Acetylhydrolase antibody pair1-Alkyl-2-Acetylglycerophosphocholine Esterase antibody pair2-Acetyl-1-Alkylglycerophosphocholine Esterase antibody pairGroup-Viia Phospholipase A2 antibody pairGviia-Pla2 antibody pairLdl-Associated Phospholipase A2 antibody pairLdl-Pla(2 antibody pairPaf 2-Acylhydrolase antibody pairPLA2G7 antibody pairPAFAH antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human LpPLA2 (Platelet-Activating Factor Acetylhydrolase) Antibody Pair (STJAP00045)
This antibody pair detects human LpPLA2, also known as Platelet-Activating Factor Acetylhydrolase, corresponding to UniProt entry Q13093. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated at 0.5 mg/mL, while the detection antibody is biotin-conjugated at 0.5 mg/mL. Both are mouse monoclonal antibodies purified by Protein A or G and formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human LpPLA2 protein. No specific immunogen region is defined in the product details.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.