Human Low-density lipoprotein receptor/LDLR protein (Recombinant) (C-His) (STJP002585)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Low-density lipoprotein receptor/LDLR Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala22-Arg788) of human LDLR/LDL Receptor (Accession #NP_000518.1) fused with a 6×His tag at
General Information
| Short Description | Recombinant-Human Low-density lipoprotein receptor/LDLR-C-His protein was developed in hek293 cells using the region Ala22-Arg788. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human LDLR at 5 Mu g/mL (100 Mu L/well) can bind Human PCSK9 with a linear range of 0.5-294.7ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | LDLR |
| Gene ID | 3949 |
| Uniprot ID | LDLR_HUMAN |
| Immunogen | Recombinant Human Low-density lipoprotein receptor/LDLR Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala22-Arg788) of human LDLR/LDL Receptor (Accession #NP_000518.1) fused with a 6×His tag at |
| Immunogen Region | Ala22-Arg788 |
| Immunogen Sequence | AVGDRCERNEFQCQDGKCIS YKWVCDGSAECQDGSDESQE TCLSVTCKSGDFSCGGRVNR CIPQFWRCDGQVDCDNGSDE QGCPPKTCSQDEFRCHDGKC ISRQFVCDSDRDCLDGSDEA SCPVLTCGPASFQCNSSTCI PQLWACDNDPDCEDGSDEWP QRCRGLYVFQGDSSPCSAFE FHCLSGECIHSSWRCDGGPD CKDKSDEENCAVATCRPDEF QCSDGNCIHGSRQCDREYD |
Additional Info
| Function | Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. (Microbial infection) Acts as a receptor for Crimean-Congo hemorrhagic fever virus (CCHFV). (Microbial infection) Acts as a receptor for many Alphavirus, including Getah virus (GETV), Ross river virus (RRV) and Semliki Forest virus. |
| Protein Name | Low-Density Lipoprotein ReceptorLdl Receptor |
| Database Links | Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8964026Reactome: R-HSA-8964038Reactome: R-HSA-975634 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembraneClathrin-Coated PitGolgi ApparatusEarly EndosomeLate EndosomeLysosomeRapidly Endocytosed Upon Ligand BindingLocalized At Cell MembraneProbably In Lipid RaftsIn Serum-Starved Conditions |
| Alternative Protein Names | Low-Density Lipoprotein Receptor proteinLdl Receptor proteinLDLR protein |
Information sourced from Uniprot.org