Human Lipocalin-2/NGAL (Lipocalin-2/NGAL) Antibody Pair (STJAP00701)
General Information
| Short Description | This Lipocalin-2/NGAL antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R09-6D-6/R09-9K-9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | LCN2 |
| Gene ID | 3934 |
| Uniprot ID | NGAL_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Detected in neutrophils (at protein level). Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Expressed in the medullary tubules of the kidney. Not found in the small intestine or peripheral blood leukocytes. |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated LipocalinNgal25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9Lipocalin-2Oncogene 24p3SiderocalinP25 |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-6799990Reactome: R-HSA-917937 |
| Cellular Localisation | SecretedCytoplasmic Granule LumenCytoplasmic Vesicle LumenUpon Binding To The Slc22a17 (24p3r) ReceptorIt Is InternalizedReleases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Antibody Pair Names | Neutrophil Gelatinase-Associated Lipocalin antibody pairNgal antibody pair25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9 antibody pairLipocalin-2 antibody pairOncogene 24p3 antibody pairSiderocalin antibody pairP25 antibody pairLCN2 antibody pairHNL antibody pairNGAL antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Lipocalin-2/NGAL (Lipocalin-2/NGAL) Antibody Pair (STJAP00701)
This antibody pair detects human Lipocalin-2/NGAL, with reactivity confirmed for human samples only. The target protein corresponds to UniProt entry P80188.
Both antibodies are rabbit monoclonal IgGs, supplied unconjugated and affinity purified. They are formulated in 0.01M PBS at pH 7.2. Clone IDs are R09-6D-6 and R09-9K-9 for the two components of the pair.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Working concentrations and dilutions are determined by the end user and are not defined in the product specifications. Titration experiments are recommended to establish optimal conditions for each specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.