Human LGR5/GPR49 protein protein (Recombinant) (C-6His) (STJP020058)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human LGR5/GPR49 protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly 22-Arg 561) of Human LGR5/GPR49 (Accession #NM_003667.3) fused with His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human LGR5/GPR49 protein-C-6His protein was developed in hek293 cells using the region Gly 22-Arg 561. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method |
Target Information
| Gene Symbol | LGR5 |
| Gene ID | 8549 |
| Uniprot ID | LGR5_HUMAN |
| Immunogen | Recombinant Human LGR5/GPR49 protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly 22-Arg 561) of Human LGR5/GPR49 (Accession #NM_003667.3) fused with His tag at the C-terminus. |
| Immunogen Region | Gly 22-Arg 561 |
| Immunogen Sequence | GSSPRSGVLLRGCPTHCHCE PDGRMLLRVDCSDLGLSELP SNLSVFTSYLDLSMNNISQL LPNPLPSLRFLEELRLAGNA LTYIPKGAFTGLYSLKVLML QNNQLRHVPTEALQNLRSLQ SLRLDANHISYVPPSCFSGL HSLRHLWLDDNALTEIPVQA FRSLSALQAMTLALNKIHHI PDYAFGNLSSLVVLHLHNNR IHSLGKKCFDGLHSLETLDL NYNNLDEFPTAIRTLSNLK |
Additional Info
| Tissue Specificity | Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. |
| Function | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. |
| Protein Name | Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5G-Protein Coupled Receptor 49G-Protein Coupled Receptor 67G-Protein Coupled Receptor Hg38 |
| Database Links | Reactome: R-HSA-4641263 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinGolgi ApparatusTrans-Golgi Network MembraneRapidly And Constitutively Internalized To The Trans-Golgi Network At Steady StateInternalization To The Trans-Golgi Network May Be The Result Of Phosphorylation At Ser-861 And Ser-864HoweverThe Phosphorylation Event Has Not Been Proven |
| Alternative Protein Names | Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5 proteinG-Protein Coupled Receptor 49 proteinG-Protein Coupled Receptor 67 proteinG-Protein Coupled Receptor Hg38 proteinLGR5 proteinGPR49 proteinGPR67 protein |
Information sourced from Uniprot.org