Human LGALS7; LGALS7B (Galectin-7) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human LGALS7; LGALS7B kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 18.75pg/mL |
| Detection Limit | 31.25~2000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | LGALS7.LGALS7B |
| Gene ID | 3963/653499 |
| Uniprot ID | LEG7_HUMAN |
| Specificity | This kit recognizes Human GAL7 in samples. No significant cross-reactivity or interference between Human GAL7 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Mainly expressed in stratified squamous epithelium. |
| Function | Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. |
| Protein Name | Galectin-7Gal-7Hkl-14Pi7P53-Induced Gene 1 Protein |
| Database Links | Reactome: R-HSA-9725554 |
| Cellular Localisation | CytoplasmNucleusSecretedMay Be Secreted By A Non-Classical Secretory Pathway |
| Alternative CLIA Names | Galectin-7 CLIA kitGal-7 CLIA kitHkl-14 CLIA kitPi7 CLIA kitP53-Induced Gene 1 Protein CLIA kitLGALS7 CLIA kitPIG1.LGALS7B CLIA kit |
| Specificity | This kit recognizes Human GAL7 in samples. No significant cross-reactivity or interference between Human GAL7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma(n=8) | 97-113 | 105 |
| Cell culture media(n=8) | 85-98 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 106.37 | 200.68 | 875.29 | 103.33 | 195.52 | 902.98 |
| Standard deviation | 13.03 | 23.64 | 81.66 | 10.09 | 22.37 | 102.67 |
| CV (%) | 12.25 | 11.78 | 9.33 | 9.76 | 11.44 | 11.37 |