Human LDLR (Low-density lipoprotein receptor) Sandwich ELISA Kit (STJE0002798)

Name: Human LDLR (Low-density lipoprotein receptor) Sandwich ELISA Kit (STJE0002798)
SKU: STJE0002798
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002798

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General Information

Short Description	This LDLR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	LDLR
Gene ID	3949
Uniprot ID	LDLR_HUMAN
Specificity	This kit recognizes Human LDLR in samples. No significant cross-reactivity or interference between Human LDLR and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Function	Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. (Microbial infection) Acts as a receptor for Crimean-Congo hemorrhagic fever virus (CCHFV). (Microbial infection) Acts as a receptor for many Alphavirus, including Getah virus (GETV), Ross river virus (RRV) and Semliki Forest virus.
Protein Name	Low-Density Lipoprotein Receptor Ldl Receptor
Database Links	Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-8964026 Reactome: R-HSA-8964038 Reactome: R-HSA-975634
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Membrane Clathrin-Coated Pit Golgi Apparatus Early Endosome Late Endosome Lysosome Rapidly Endocytosed Upon Ligand Binding Localized At Cell Membrane Probably In Lipid Rafts In Serum-Starved Conditions
Alternative ELISA Names	Low-Density Lipoprotein Receptor ELISA kit Ldl Receptor ELISA kit LDLR ELISA kit
Specificity	This kit recognizes Human LDLR in samples. No significant cross-reactivity or interference between Human LDLR and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-108	98
EDTA plasma (n=8)	87-103	94
Cell culture media (n=8)	94-105	100

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.01	1.70	7.95	0.95	1.81	8.59
Standard deviation	0.05	0.09	0.38	0.05	0.08	0.37
CV (%)	4.95	5.29	4.78	5.26	4.42	4.31

Citations

View product citations for antibody STJE0002798 on CiteAb