Human LBP (Lipopolysaccharide-binding protein) CLIA Kit for Quantitative Detection

SPECIFICATIONS
STJC0000939
🚚 Free UK Delivery on orders over £150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionThis human LBP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
ApplicationsCLIA
ReactivityHuman
Sensitivity0.38ng/mL
Detection Limit0.63~40ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionChemiluminescence

Target Information

Gene SymbolLBP
Gene ID3929
Uniprot IDLBP_HUMAN
SpecificityThis kit recognizes Human LBP in samples. No significant cross-reactivity or interference between Human LBP and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Tissue Specificity Detected in blood serum (at protein level).
Function Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Protein Name Lipopolysaccharide-Binding Protein
Lbp
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-166020
Reactome: R-HSA-5686938
Reactome: R-HSA-6785807
Cellular Localisation Secreted
Cytoplasmic Granule Membrane
Membrane-Associated In Polymorphonuclear Leukocytes (Pmn) Granules
Alternative CLIA Names Lipopolysaccharide-Binding Protein CLIA kit
Lbp CLIA kit
LBP CLIA kit
Specificity This kit recognizes Human LBP in samples. No significant cross-reactivity or interference between Human LBP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-113 106
EDTA plasma(n=8) 97-110 104
Cell culture media(n=8) 92-104 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.88 4.39 19.45 1.73 4.50 18.42
Standard deviation 0.20 0.46 1.87 0.19 0.35 1.27
CV (%) 10.64 10.48 9.61 10.98 7.78 6.89

Citations

Product Review

Well-cited Academic Publications
KO-Validated 700+ Lines
Technical Support Expert Scientific Team
1-Year Guarantee Antibodies & Proteins