Human KRT10 (Keratin, type I cytoskeletal 10) CLIA Kit for Quantitative Detection

Name: Human KRT10 (Keratin, type I cytoskeletal 10) CLIA Kit for Quantitative Detection
SKU: STJC0000893
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000893

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

Short Description	This human KRT10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	4.69pg/mL
Detection Limit	7.81~500pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	KRT10
Gene ID	3858
Uniprot ID	K1C10_HUMAN
Specificity	This kit recognizes Human KRT10 in samples. No significant cross-reactivity or interference between Human KRT10 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines. Localized on the surface of desquamated nasal epithelial cells (at protein level).
Function	Plays a role in the establishment of the epidermal barrier on plantar skin. Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
Protein Name	Keratin - Type I Cytoskeletal 10 Cytokeratin-10 Ck-10 Keratin-10 K10
Database Links	Reactome: R-HSA-6805567 Reactome: R-HSA-6809371 Reactome: R-HSA-9725554
Cellular Localisation	Secreted Extracellular Space Cell Surface Cytoplasm
Alternative CLIA Names	Keratin - Type I Cytoskeletal 10 CLIA kit Cytokeratin-10 CLIA kit Ck-10 CLIA kit Keratin-10 CLIA kit K10 CLIA kit KRT10 CLIA kit KPP CLIA kit
Specificity	This kit recognizes Human KRT10 in samples. No significant cross-reactivity or interference between Human KRT10 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	27.48	65.37	171.25	27.44	67.27	165.60
Standard deviation	2.70	6.92	10.28	3.36	5.11	12.04
CV (%)	9.83	10.59	6.00	12.24	7.60	7.27