Human KPNB1 protein (Recombinant) (N-His) (STJP014632)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human KPNB1-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Supplied as a 0.22 Mu m filtered solution in 20mM Tris-HCl, 1mM DTT, 30% Glycerol, 100mM NaCl, pH 8.0. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | KPNB1 |
| Gene ID | 3837 |
| Uniprot ID | IMB1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Ala876 (Q559H) |
Additional Info
| Function | Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. In association with IPO7, mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Imports MRTFA, SNAI1 and PRKCI into the nucleus. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. |
| Protein Name | Importin Subunit Beta-1Importin-90Karyopherin Subunit Beta-1Nuclear Factor P97Pore Targeting Complex 97 Kda SubunitPtac97 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-140342Reactome: R-HSA-1655829Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-180746Reactome: R-HSA-2995383Reactome: R-HSA-6798695Reactome: R-HSA-68616Reactome: R-HSA-909733Reactome: R-HSA-9615933Reactome: R-HSA-9636249Reactome: R-HSA-9692916 |
| Cellular Localisation | CytoplasmNucleus Envelope |
| Alternative Protein Names | Importin Subunit Beta-1 proteinImportin-90 proteinKaryopherin Subunit Beta-1 proteinNuclear Factor P97 proteinPore Targeting Complex 97 Kda Subunit proteinPtac97 proteinKPNB1 proteinNTF97 protein |
Information sourced from Uniprot.org