Human KL (Klotho) Sandwich ELISA Kit (STJE0003848)
SPECIFICATIONS
General Information
| Short Description | This KL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.19ng/mL |
| Detection Limit | 0.31~20ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | KL |
| Gene ID | 9365 |
| Uniprot ID | KLOT_HUMAN |
| Specificity | This kit recognizes Human KL in samples. No significant cross-reactivity or interference between Human KL and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. |
| Protein Name | Klotho Cleaved Into - Klotho Peptide |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-190374Reactome: R-HSA-2219530Reactome: R-HSA-5654219Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654726Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinApical Cell MembraneIsoform 1 Shedding Leads To A Soluble PeptideIsoform 2: SecretedKlotho Peptide: Secreted |
| Alternative ELISA Names | Klotho Cleaved Into - Klotho Peptide ELISA kitKL ELISA kit |
| Specificity | This kit recognizes Human KL in samples. No significant cross-reactivity or interference between Human KL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-99 | 91 |
| EDTA plasma (n=8) | 89-102 | 96 |
| Cell culture media (n=8) | 85-98 | 90 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.04 | 2.05 | 9.74 | 0.97 | 2.04 | 9.08 |
| Standard deviation | 0.06 | 0.11 | 0.34 | 0.05 | 0.10 | 0.48 |
| CV (%) | 5.77 | 5.37 | 3.49 | 5.15 | 4.90 | 5.29 |