| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.107ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This KCC2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | SLC12A5 |
| Gene ID: | 57468 |
| Uniprot ID: | S12A5_HUMAN |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Brain specific. Detected in neuronal cells. |
| Post Translational Modifications | Phosphorylated at Thr-929 and Thr-1030 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. |
| Function | Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation. |
| Protein Name | Solute Carrier Family 12 Member 5 Electroneutral Potassium-Chloride Cotransporter 2 K-Cl Cotransporter 2 Hkcc2 Neuronal K-Cl Cotransporter |
| Database Links | Reactome: R-HSA-426117 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Cell Projection Dendrite Detected On Dendrites But Not On Axons Of Spinal Cord Neurons And At Gphn-Positive Inhibitory Synapses |
| Alternative ELISA Names | Solute Carrier Family 12 Member 5 ELISA kit Electroneutral Potassium-Chloride Cotransporter 2 ELISA kit K-Cl Cotransporter 2 ELISA kit Hkcc2 ELISA kit Neuronal K-Cl Cotransporter ELISA kit SLC12A5 ELISA kit KCC2 ELISA kit KIAA1176 ELISA kit |
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Information sourced from Uniprot.org
