Human Kallikrein-11 protein (Recombinant) (STJP000703)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Kallikrein-11-hK11 (GLU19-ASN250) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Kallikrein-11-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by the ability to cleave a fluorogenic substrate VLK-SBzl.The specific activity was measured to be 300 pmole/ug/min NA |
Target Information
| Gene Symbol | KLK11 |
| Gene ID | 11012 |
| Uniprot ID | KLK11_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Kallikrein-11-hK11 (GLU19-ASN250) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | GLU19-ASN250 |
Additional Info
| Tissue Specificity | Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). |
| Post Translational Modifications | About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen. |
| Function | Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. |
| Protein Name | Kallikrein-11Hk11HippostasinSerine Protease 20Trypsin-Like Protease Cleaved Into - Kallikrein-11 Inactive Chain 1 - Kallikrein-11 Inactive Chain 2 |
| Database Links | |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Golgi ApparatusSecreted |
| Alternative Protein Names | Kallikrein-11 proteinHk11 proteinHippostasin proteinSerine Protease 20 proteinTrypsin-Like Protease Cleaved Into - Kallikrein-11 Inactive Chain 1 - Kallikrein-11 Inactive Chain 2 proteinKLK11 proteinPRSS20 proteinTLSP proteinUNQ649 proteinPRO1279 protein |
Information sourced from Uniprot.org