Human IZUMO1 protein (Recombinant) (C-Strep) (STJP010145)
SPECIFICATIONS
HostMammalian Cells
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human IZUMO1-C-Strep protein was developed from mammalian cells for the region C-Strep. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | IZUMO1 |
| Gene ID | 284359 |
| Uniprot ID | IZUM1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Cys22-Pro254 |
Additional Info
| Post Translational Modifications | N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm. Phosphorylated. The cytoplasmic C-terminus is phosphorylated and undergoes phosphorylation changes during epididymal transit. |
| Function | Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization. FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion. Plays a critical role in sperm-oolemma binding prior to plasma membrane fusion. Can mediate cell-cell fusion in cultured mammalian cells independently of its binding to IZUMO1R/JUNO. |
| Protein Name | Izumo Sperm-Egg Fusion Protein 1Oocyte Binding/Fusion FactorObfSperm-Specific Protein Izumo |
| Database Links | Reactome: R-HSA-1300645 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmic VesicleSecretory VesicleAcrosome MembraneLocalizes Initially To The Acrosome Membrane Of The Sperm Head (Both Outer And Inner Acrosomal Membranes)During The Acrosome ReactionTranslocates To The Plasma Membrane |
| Alternative Protein Names | Izumo Sperm-Egg Fusion Protein 1 proteinOocyte Binding/Fusion Factor proteinObf proteinSperm-Specific Protein Izumo proteinIZUMO1 protein |
Information sourced from Uniprot.org