Human IP-10/CXCL10 (C-X-C Motif Chemokine 10) Antibody Pair (STJAP00187)
General Information
| Short Description | This IP-10/CXCL10 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A & Antigen Affinity.Detection Purification: Protein A & Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CXCL10 |
| Gene ID | 3627 |
| Uniprot ID | CXL10_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human IP-10/CXCL10 protein.Detection Immunogen: Recombinant Human IP-10/CXCL10 protein. |
Additional Info
| Post Translational Modifications | Several proteases can mediate post-secretion cleavages. DPP4 cleaves CXCL10 on its N-terminal 2 amino acids leading to an antagonist form of CXCL10. This dominant negative form is capable of binding CXCR3 but does not induce signaling. MMP9 cleaves 9 amino acids instead. |
| Function | Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization. |
| Protein Name | C-X-C Motif Chemokine 1010 Kda Interferon Gamma-Induced ProteinGamma-Ip10Ip-10Small-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | C-X-C Motif Chemokine 10 antibody pair10 Kda Interferon Gamma-Induced Protein antibody pairGamma-Ip10 antibody pairIp-10 antibody pairSmall-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73 antibody pairCXCL10 antibody pairINP10 antibody pairSCYB10 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IP-10/CXCL10 (C-X-C Motif Chemokine 10) Antibody Pair (STJAP00187)
This antibody pair detects human IP-10/CXCL10 (C-X-C Motif Chemokine 10), corresponding to UniProt entry P02778. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit polyclonal antibodies supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant human IP-10/CXCL10 protein. Both antibodies were purified using Protein A and antigen affinity methods.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.