Human Interleukin-7 protein (Recombinant) (STJP000231)
SPECIFICATIONS
HostCHO Cells
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Interleukin-7 mature chain was expressed in CHO Cells. NA
General Information
| Short Description | Recombinant-Human Interleukin-7-protein was developed from cho cells. For use in research applications. |
| Host | CHO Cells |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution pH7.5. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The ED (50) determinedby dose-dependent stimulation of the proliferation of murineE8 cells is ≤.5 ng/ml, corresponding to a specific activity of ≥ x units/mg. NA |
Target Information
| Gene Symbol | IL7 |
| Gene ID | 3574 |
| Uniprot ID | IL7_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Interleukin-7 mature chain was expressed in CHO Cells. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | MFHVSFRYIF GLPPLILVLL PVASSDCDIE GKDGKQYESV LMVSIDQLLD SMKEIGSNCL NNEFNFFKRH ICDANKEGMF LFRAARKLRQ FLKMNSTGDF DLHLLKVSEG TTILLNCTGQ VKGRKPAALG EAQPTKSLEE NKSLKEQKKL NDLCFLKRLL QEIKTCWNKI LMGTKEH NA |
Additional Info
| Function | Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5. |
| Protein Name | Interleukin-7Il-7 |
| Database Links | Reactome: R-HSA-1266695 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-7 proteinIl-7 proteinIL7 protein |
Information sourced from Uniprot.org