Human Interleukin-2 protein (Recombinant) (STJP000240)
SPECIFICATIONS
HostCHO Cells
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Interleukin-2 mature chain was expressed in CHO Cells. NA
General Information
| Short Description | Recombinant-Human Interleukin-2-protein was developed from cho cells. For use in research applications. |
| Host | CHO Cells |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered concentrated (1mg/ml) solution in PBS, pH.2. |
| Storage Instruction | Can be stored in working aliquots atΒ°C-Β°C C for one month, or at-20Β°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/Β΅g (1EU/Β΅g). NA |
| Immunoreactivity | The ED (50) was determined by the dose dependent proliferation of murine CTLL-2 cells andwasfound to be less than.1 ng/ml., corresponding to a specific activity of >2.0x units/mg. NA |
Target Information
| Gene Symbol | IL2 |
| Gene ID | 3558 |
| Uniprot ID | IL2_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Interleukin-2 mature chain was expressed in CHO Cells. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | MYRMQLLSCI ALSLALVTNS APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFAQSIIS TLT NA |
Additional Info
| Function | Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells. |
| Protein Name | Interleukin-2Il-2T-Cell Growth FactorTcgfAldesleukin |
| Database Links | Reactome: R-HSA-5673001Reactome: R-HSA-8877330Reactome: R-HSA-9020558Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-2 proteinIl-2 proteinT-Cell Growth Factor proteinTcgf proteinAldesleukin proteinIL2 protein |
Information sourced from Uniprot.org