| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Interleukin-15-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Biotin |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution pH7.5. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
Activity was determined by the binding of human IL-15 receptor alpha chain in a functional ELISA assay in a linear range of 1-100 ng/ml. NA |
| Gene Symbol: |
IL15 |
| Gene ID: |
3600 |
| Uniprot ID: |
IL15_HUMAN |
| Immunogen Region: |
Mature chain |
| Immunogen: |
DNA sequence encoding Human Interleukin-15 mature chain fused to an AVI IgG1 C-terminal tag was expressed in HEK292 cells. NA |
| Tissue Specificity | Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. |
| Function | Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines. In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection. Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5. In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4. |
| Protein Name | Interleukin-15Il-15 |
| Database Links | Reactome: R-HSA-8983432 |
| Cellular Localisation | Isoform Il15-S48aa: SecretedIsoform Il15-S21aa: CytoplasmNucleusIl15-S21aa Is Not SecretedBut Rather Is Stored IntracellularlyAppearing In The Nucleus And Cytoplasmic Components |
| Alternative Protein Names | Interleukin-15 proteinIl-15 proteinIL15 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
