• Biotinylated Human ITGA5&ITGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC.
  • Immobilized 0. 5 Mu g Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0. 5 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55. 3ng/ml determined by ELISA.

Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer protein (Recombinant) (C-His-Avi) {Biotin} (STJP001546)

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STJP001546

€840.63 - €2,615.63
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: ITGA5
Gene ID: 3678
Uniprot ID: ITA5_HUMAN
Immunogen Region: Phe42-Tyr995 (ITGA5) acidic tail & Gln21-Asp728 (ITGB1) basic tail
Immunogen: DNA sequence encoding Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Proteolytic cleavage by PCSK5 mediates activation of the precursor.
Function Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Protein Name Integrin Alpha-5
Cd49 Antigen-Like Family Member E
Fibronectin Receptor Subunit Alpha
Integrin Alpha-F
Vla-5
Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-1566977
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-445144
Reactome: R-HSA-8941332
Reactome: R-HSA-9634597
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Focal Adhesion
Alternative Protein Names Integrin Alpha-5 protein
Cd49 Antigen-Like Family Member E protein
Fibronectin Receptor Subunit Alpha protein
Integrin Alpha-F protein
Vla-5 protein
Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain protein
ITGA5 protein
FNRA protein

Information sourced from Uniprot.org

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