Human INS (Insulin) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human INS kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 0.38ng/mL |
| Detection Limit | 0.63~40ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | INS |
| Gene ID | 3630 |
| Uniprot ID | INS_HUMAN |
| Specificity | This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. |
| Protein Name | Insulin Cleaved Into - Insulin B Chain - Insulin A Chain |
| Database Links | Reactome: R-HSA-210745Reactome: R-HSA-264876Reactome: R-HSA-422085Reactome: R-HSA-422356Reactome: R-HSA-6807878Reactome: R-HSA-6811558Reactome: R-HSA-74713Reactome: R-HSA-74749Reactome: R-HSA-74751Reactome: R-HSA-74752Reactome: R-HSA-77387Reactome: R-HSA-9615017Reactome: R-HSA-9768919Reactome: R-HSA-977225 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Insulin Cleaved Into - Insulin B Chain - Insulin A Chain CLIA kitINS CLIA kit |
| Specificity | This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-101 | 96 |
| EDTA plasma(n=8) | 85-99 | 92 |
| Cell culture media(n=8) | 97-112 | 103 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.83 | 6.14 | 14.27 | 1.98 | 6.69 | 14.09 |
| Standard deviation | 0.19 | 0.56 | 0.94 | 0.23 | 0.74 | 0.92 |
| CV (%) | 10.38 | 9.12 | 6.59 | 11.62 | 11.06 | 6.53 |