Human ILK protein (Recombinant) (N-His) (STJP005325)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ILK-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ILK |
| Gene ID | 3611 |
| Uniprot ID | ILK_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asn183-Lys452 |
Additional Info
| Post Translational Modifications | Phosphorylation by PAK1 modulates ILK subcellular location by promoting its nuclear export. |
| Function | Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes. Acts as an upstream effector of both AKT1/PKB and GSK3. Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA. Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus. |
| Protein Name | Scaffold Protein IlkIlk-1Ilk-2Inactive Integrin-Linked KinaseP59ilk |
| Database Links | Reactome: R-HSA-446343Reactome: R-HSA-446353 |
| Cellular Localisation | Cell JunctionFocal AdhesionCell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionLamellipodiumCytoplasmMyofibrilSarcomereNucleusCytoskeletonMicrotubule Organizing CenterCentrosomeCell Cortex |
| Alternative Protein Names | Scaffold Protein Ilk proteinIlk-1 proteinIlk-2 proteinInactive Integrin-Linked Kinase proteinP59ilk proteinILK proteinILK1 proteinILK2 protein |
Information sourced from Uniprot.org