Human IL34 (Interleukin-34) Sandwich ELISA Kit (STJE0003131)

Name: Human IL34 (Interleukin-34) Sandwich ELISA Kit (STJE0003131)
SKU: STJE0003131
Availability: InStock

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STJE0003131

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Short Description	This IL34 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	56.25pg/mL
Detection Limit	93.75~6000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	IL34
Gene ID	146433
Uniprot ID	IL34_HUMAN
Specificity	This kit recognizes Human IL-34 in samples. No significant cross-reactivity or interference between Human IL-34 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
Protein Name	Interleukin-34 Il-34
Database Links	Reactome: R-HSA-449836 Reactome: R-HSA-9680350
Cellular Localisation	Secreted
Alternative ELISA Names	Interleukin-34 ELISA kit Il-34 ELISA kit IL34 ELISA kit C16orf77 ELISA kit
Specificity	This kit recognizes Human IL-34 in samples. No significant cross-reactivity or interference between Human IL-34 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	292.00	719.71	2331.63	295.64	706.23	2493.50
Standard deviation	15.07	38.22	90.47	15.46	39.62	112.71
CV (%)	5.16	5.31	3.88	5.23	5.61	4.52