This Interleukin-18 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and cell culture supernates.
Applications
ELISA
Reactivity
Human
Sensitivity
12 pg/mL
Detection Limit
234-1500 pg/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Conjugation
Biotin
Storage Instruction
Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Isoform 2: Expressed in ovarian carcinoma but undetectable in normal ovarian epithelial cells. Resistant to proteolytic activation by caspase-1 and -4.
Post Translational Modifications
The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18.
Function
Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
CytoplasmCytosolSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1Casp4 Or Casp5 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
