Human IL-8 (Interleukin-8) Antibody Pair (STJAP00041)
General Information
| Short Description | This IL-8 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CXCL8 |
| Gene ID | 3576 |
| Uniprot ID | IL8_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human IL-8 protein.Detection Immunogen: Recombinant Human IL-8 protein. |
Additional Info
| Function | Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. |
| Protein Name | Interleukin-8Il-8C-X-C Motif Chemokine 8ChemokineC-X-C Motif Ligand 8EmoctakinGranulocyte Chemotactic Protein 1Gcp-1Monocyte-Derived Neutrophil Chemotactic FactorMdncfMonocyte-Derived Neutrophil-Activating PeptideMonapNeutrophil-Activating Protein 1Nap-1Protein 3-10cT-Cell Chemotactic Factor Cleaved Into - Mdncf-AGcp/Il-8 Protein IvIl8/Nap1 Form I - Interleukin-8(Ala-Il-877Gcp/Il-8 Protein IiIl-8(1-77Il8/Nap1 Form IiMdncf-B - Il-8(5-77 - Il-8(6-77(Ser-Il-872Gcp/Il-8 Protein IIl8/Nap1 Form IiiLymphocyte-Derived Neutrophil-Activating FactorLynapMdncf-CNeutrophil-Activating FactorNaf - Il-8(7-77Gcp/Il-8 Protein VIl8/Nap1 Form Iv - Il-8(8-77Gcp/Il-8 Protein ViIl8/Nap1 Form V - Il-8(9-77Gcp/Il-8 Protein IiiIl8/Nap1 Form Vi |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-375276Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-418594Reactome: R-HSA-6783783Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-8 antibody pairIl-8 antibody pairC-X-C Motif Chemokine 8 antibody pairChemokine antibody pairC-X-C Motif Ligand 8 antibody pairEmoctakin antibody pairGranulocyte Chemotactic Protein 1 antibody pairGcp-1 antibody pairMonocyte-Derived Neutrophil Chemotactic Factor antibody pairMdncf antibody pairMonocyte-Derived Neutrophil-Activating Peptide antibody pairMonap antibody pairNeutrophil-Activating Protein 1 antibody pairNap-1 antibody pairProtein 3-10c antibody pairT-Cell Chemotactic Factor Cleaved Into - Mdncf-A antibody pairGcp/Il-8 Protein Iv antibody pairIl8/Nap1 Form I - Interleukin-8 antibody pair(Ala-Il-877 antibody pairGcp/Il-8 Protein Ii antibody pairIl-8(1-77 antibody pairIl8/Nap1 Form Ii antibody pairMdncf-B - Il-8(5-77 - Il-8(6-77 antibody pair(Ser-Il-872 antibody pairGcp/Il-8 Protein I antibody pairIl8/Nap1 Form Iii antibody pairLymphocyte-Derived Neutrophil-Activating Factor antibody pairLynap antibody pairMdncf-C antibody pairNeutrophil-Activating Factor antibody pairNaf - Il-8(7-77 antibody pairGcp/Il-8 Protein V antibody pairIl8/Nap1 Form Iv - Il-8(8-77 antibody pairGcp/Il-8 Protein Vi antibody pairIl8/Nap1 Form V - Il-8(9-77 antibody pairGcp/Il-8 Protein Iii antibody pairIl8/Nap1 Form Vi antibody pairCXCL8 antibody pairIL8 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-8 (Interleukin-8) Antibody Pair (STJAP00041)
This antibody pair detects human IL-8 (Interleukin-8, UniProt P10145) and has confirmed reactivity with human samples only. Both the capture and detection antibodies were raised against recombinant human IL-8 protein.
The capture antibody is an unconjugated mouse monoclonal antibody purified by Protein A or G, supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4. The detection antibody is a biotin-conjugated goat polyclonal antibody purified by antigen affinity, supplied at the same concentration and in an identical buffer formulation.
This antibody pair is validated for use in ELISA only. No other assay formats are listed in the product specifications.
The capture antibody was purified by Protein A or G, and the detection antibody was purified by antigen affinity chromatography. This combination is typical for sandwich ELISA configurations, and the product is validated for ELISA use.
Both antibodies should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided. The glycerol content in the formulation is consistent with storage at this temperature.
No. This product is for research use only and is not intended for diagnostic, therapeutic, or clinical procedures.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.