Human IL-7 protein (Recombinant) (C-His) (STJP001120)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenActive Recombinant Human IL-7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp26-His177) of human IL-7 (Accession #NP_000871.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human IL-7-C-His protein was developed in hek293 cells using the region Asp26-His177. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA.Immobilized Human IL7 at 2 Mu g/mL (100 Mu L/well) can bind human IL7R with a linear range of 0.97-159.1 ng/mL.|2.Measured in a cell proliferation assay using IL-2-activated mouse spleen cell. T |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL7 |
| Gene ID | 3574 |
| Uniprot ID | IL7_HUMAN |
| Immunogen | Active Recombinant Human IL-7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp26-His177) of human IL-7 (Accession #NP_000871.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Asp26-His177 |
| Immunogen Sequence | DCDIEGKDGKQYESVLMVSI DQLLDSMKEIGSNCLNNEFN FFKRHICDANKEGMFLFRAA RKLRQFLKMNSTGDFDLHLL KVSEGTTILLNCTGQVKGRK PAALGEAQPTKSLEENKSLK EQKKLNDLCFLKRLLQEIKT CWNKILMGTKEH |
Additional Info
| Function | Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5. |
| Protein Name | Interleukin-7Il-7 |
| Database Links | Reactome: R-HSA-1266695 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-7 proteinIl-7 proteinIL7 protein |
Information sourced from Uniprot.org