Human IL-5 (IL-5) Antibody Pair (STJAP00726)
General Information
| Short Description | This IL-5 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R04-9V9/R04-9E2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL5 |
| Gene ID | 3567 |
| Uniprot ID | IL5_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Present in peripheral blood mononuclear cells. |
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5Il-5B-Cell Differentiation Factor IEosinophil Differentiation FactorT-Cell Replacing FactorTrf |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-5 antibody pairIl-5 antibody pairB-Cell Differentiation Factor I antibody pairEosinophil Differentiation Factor antibody pairT-Cell Replacing Factor antibody pairTrf antibody pairIL5 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-5 (IL-5) Antibody Pair (STJAP00726)
This antibody pair is designed to detect human Interleukin-5 (IL-5), corresponding to UniProt accession P05113. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit monoclonal IgGs, supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. Both have been affinity purified, and clone IDs are R04-9V9 and R04-9E2 respectively.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the current product data.
Optimal working concentrations are determined by the end user and are not specified in the product data. It is recommended to perform a titration experiment to establish the appropriate dilution for your specific assay conditions.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Both antibodies in this pair were raised against a recombinant human IL-5 protein. No further details regarding the specific immunogen region are provided in the current product data.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.