Human IL-4 protein (Recombinant) (No-tag) (STJP020080)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His25-Ser153) of human IL-4 (Accession #NP_000580.1) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Human IL-4-No-tag protein was developed in hek293 cells using the region His25-Ser153. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL4 |
| Gene ID | 3565 |
| Uniprot ID | IL4_HUMAN |
| Immunogen | Recombinant Human IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His25-Ser153) of human IL-4 (Accession #NP_000580.1) fused with no additional amino acid. |
| Immunogen Region | His25-Ser153 |
| Immunogen Sequence | HKCDITLQEIIKTLNSLTEQ KTLCTELTVTDIFAASKNTT EKETFCRAATVLRQFYSHHE KDTRCLGATAQQFHRHKQLI RFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIM REKYSKCSS |
Additional Info
| Function | Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6. |
| Protein Name | Interleukin-4Il-4B-Cell Stimulatory Factor 1Bsf-1BinetrakinLymphocyte Stimulatory Factor 1Pitrakinra |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9012546 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-4 proteinIl-4 proteinB-Cell Stimulatory Factor 1 proteinBsf-1 proteinBinetrakin proteinLymphocyte Stimulatory Factor 1 proteinPitrakinra proteinIL4 protein |
Information sourced from Uniprot.org