Human IL-4 (IL-4) Antibody Pair (STJAP00725)
General Information
| Short Description | This IL-4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R03-4E8/R08-7K8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL4 |
| Gene ID | 3565 |
| Uniprot ID | IL4_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Function | Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6. |
| Protein Name | Interleukin-4Il-4B-Cell Stimulatory Factor 1Bsf-1BinetrakinLymphocyte Stimulatory Factor 1Pitrakinra |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9012546 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-4 antibody pairIl-4 antibody pairB-Cell Stimulatory Factor 1 antibody pairBsf-1 antibody pairBinetrakin antibody pairLymphocyte Stimulatory Factor 1 antibody pairPitrakinra antibody pairIL4 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-4 (IL-4) Antibody Pair (STJAP00725)
This antibody pair is designed to detect human Interleukin-4 (IL-4), corresponding to UniProt entry P05112. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. Both have been affinity purified, with clone IDs R03-4E8 and R08-7K8 for the pair.
This antibody pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both antibodies were raised against a recombinant protein immunogen corresponding to human IL-4. No further detail on the immunogen region is provided in the current product specification.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles.
Working dilutions are to be determined by the end user and are not specified in the product datasheet. Titration is recommended to establish optimal concentrations for each specific experimental setup.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.