Human IL-3RA/CD123 protein (Recombinant) (C-His&Avi) {Biotin} (STJP019905)
SPECIFICATIONS
HostHEK293 cells
ConjugationBiotin
ImmunogenBiotinylated Recombinant Human IL-3RA/CD123 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr19-Arg305) of Human IL-3 R alpha/CD123 (Accession #P26951-1) fused with a C-His&Avi tag at the C-term
General Information
| Short Description | Recombinant-Human IL-3RA/CD123-C-His&Avi protein was developed in hek293 cells using the region Thr19-Arg305. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL3RA |
| Gene ID | 3563 |
| Uniprot ID | IL3RA_HUMAN |
| Immunogen | Biotinylated Recombinant Human IL-3RA/CD123 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr19-Arg305) of Human IL-3 R alpha/CD123 (Accession #P26951-1) fused with a C-His&Avi tag at the C-term |
| Immunogen Region | Thr19-Arg305 |
| Immunogen Sequence | TKEDPNPPITNLRMKAKAQQ LTWDLNRNVTDIECVKDADY SMPAVNNSYCQFGAISLCEV TNYTVRVANPPFSTWILFPE NSGKPWAGAENLTCWIHDVD FLSCSWAVGPGAPADVQYDL YLNVANRRQQYECLHYKTDA QGTRIGCRFDDISRLSSGSQ SSHILVRGRSAAFGIPCTDK FVVFSQIEILTPPNMTAKCN KTHSFMHWKMRSHFNRKFRY ELQIQKRMQPVITEQVRDR |
Additional Info
| Post Translational Modifications | Ubiquitinated by RNFT2 in response to IL3. Ubiquitination leads ligand-induced degradation by the proteasome. Ubiquitinated by RNF128 via 'Lys-27'-linked polyubiquitination, facilitating its degradation through the lysosomal pathway. |
| Function | Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program. |
| Protein Name | Interleukin-3 Receptor Subunit AlphaIl-3 Receptor Subunit AlphaIl-3r Subunit AlphaIl-3r-AlphaIl-3raCd Antigen Cd123 |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-912526 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Interleukin-3 Receptor Subunit Alpha proteinIl-3 Receptor Subunit Alpha proteinIl-3r Subunit Alpha proteinIl-3r-Alpha proteinIl-3ra proteinCd Antigen Cd123 proteinIL3RA proteinIL3R protein |
Information sourced from Uniprot.org