Human IL-33 (IL-33) Antibody Pair (STJAP00886)
General Information
| Short Description | This IL-33 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R06-7P-2/R03-3L-6 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL33 |
| Gene ID | 90865 |
| Uniprot ID | IL33_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. |
| Post Translational Modifications | The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase. |
| Function | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation. |
| Protein Name | Interleukin-33Il-33Interleukin-1 Family Member 11Il-1f11Nuclear Factor From High Endothelial VenulesNf-Hev Cleaved Into - Interleukin-3395-270 - Interleukin-3399-270 - Interleukin-33109-270 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-5689880Reactome: R-HSA-6811558Reactome: R-HSA-9014843 |
| Cellular Localisation | NucleusChromosomeCytoplasmCytoplasmic VesicleSecretory VesicleSecretedSecreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore Following Cleavage By Cela1Associates With Heterochromatin And Mitotic ChromosomesThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Pair Names | Interleukin-33 antibody pairIl-33 antibody pairInterleukin-1 Family Member 11 antibody pairIl-1f11 antibody pairNuclear Factor From High Endothelial Venules antibody pairNf-Hev Cleaved Into - Interleukin-33 antibody pair95-270 - Interleukin-33 antibody pair99-270 - Interleukin-33 antibody pair109-270 antibody pairIL33 antibody pairC9orf26 antibody pairIL1F11 antibody pairNFHEV antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-33 (IL-33) Antibody Pair (STJAP00886)
This antibody pair is designed to detect human IL-33 (UniProt O95760). Reactivity has been confirmed for human samples only.
This antibody pair is validated for use in ELISA. It has not been validated for other assay formats based on the available product data.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated in 0.01M PBS at pH 7.2. Clone IDs are R06-7P-2 and R03-3L-6, and both are purified by affinity purification.
Working concentrations are not pre-defined for this pair and must be determined by the end user through titration experiments. This is standard practice for sandwich ELISA development with unlabeled antibody pairs.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided.
Both antibodies in this pair were raised against a recombinant protein immunogen corresponding to human IL-33. No specific immunogen region data is provided in the current product information.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.