Human IL-3 (IL-3) Antibody Pair (STJAP00935)
General Information
| Short Description | This IL-3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | IL3 |
| Gene ID | 3562 |
| Uniprot ID | IL3_HUMAN |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK. |
| Protein Name | Interleukin-3Il-3Hematopoietic Growth FactorMast Cell Growth FactorMcgfMultipotential Colony-Stimulating FactorP-Cell-Stimulating Factor |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-8939247Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-3 antibody pairIl-3 antibody pairHematopoietic Growth Factor antibody pairMast Cell Growth Factor antibody pairMcgf antibody pairMultipotential Colony-Stimulating Factor antibody pairP-Cell-Stimulating Factor antibody pairIL3 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-3 (IL-3) Antibody Pair (STJAP00935)
This antibody pair is designed to detect human IL-3 (Interleukin-3), corresponding to UniProt entry P08700. Reactivity has been confirmed for human samples only.
The pair consists of two mouse-derived antibodies: one unconjugated antibody supplied at 0.5 mg/mL, which serves as the capture antibody, and one biotin-conjugated antibody supplied at 0.5 to 4 ug/mL for use as the detection antibody.
This pair has been validated for use in sandwich ELISA with colorimetric detection. No other assay formats are listed in the product specifications.
This antibody pair has been validated for use with serum, plasma, and other biological fluids. Researchers should optimize conditions for any sample type not explicitly listed.
The recommended dilution range for this pair is 1:1000 to 1:10000. Researchers should perform titration experiments to determine the optimal concentration for their specific assay conditions.
Both antibodies should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.