human IL-25 protein (Recombinant) (C-6His) (STJP001931)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant human IL-25 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr33-Gly177) of human IL-25 (Accession #NP_073626.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-human IL-25-C-6His protein was developed in hek293 cells using the region Tyr33-Gly177. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 8.0 |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 13.32-53.24 ng/mL, corresponding to a specific activity of 1.88×104~7.51×104 units/mg. |
| Determination Method | < 0.1 EU/Mu g |
Target Information
| Gene Symbol | IL25 |
| Gene ID | 64806 |
| Uniprot ID | IL25_HUMAN |
| Immunogen | Recombinant human IL-25 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr33-Gly177) of human IL-25 (Accession #NP_073626.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Tyr33-Gly177 |
| Immunogen Sequence | YSHWPSCCPSKGQDTSEELL RWSTVPVPPLEPARPNRHPE SCRASEDGPLNSRAISPWRY ELDRDLNRLPQDLYHARCLC PHCVSLQTGSHMDPRGNSEL LYHNQTVFYRRPCHGEKGTH KGYCLERRLYRVSLACVCVR PRVMG |
Additional Info
| Function | Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Also induces the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways. Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13. |
| Protein Name | Interleukin-25Il-25Interleukin-17eIl-17e |
| Database Links | Reactome: R-HSA-448424 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-25 proteinIl-25 proteinInterleukin-17e proteinIl-17e proteinIL25 proteinIL17E proteinUNQ3120 proteinPRO10272 protein |
Information sourced from Uniprot.org