Human IL-25 (IL-25) Antibody Pair (STJAP00885)
General Information
| Short Description | This IL-25 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R08-4U-7/R06-7D-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL25 |
| Gene ID | 64806 |
| Uniprot ID | IL25_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea. |
| Function | Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Also induces the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways. Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13. |
| Protein Name | Interleukin-25Il-25Interleukin-17eIl-17e |
| Database Links | Reactome: R-HSA-448424 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-25 antibody pairIl-25 antibody pairInterleukin-17e antibody pairIl-17e antibody pairIL25 antibody pairIL17E antibody pairUNQ3120 antibody pairPRO10272 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-25 (IL-25) Antibody Pair (STJAP00885)
This antibody pair detects human IL-25 (also designated IL-17E), corresponding to UniProt entry Q9H293. Reactivity is confirmed for human samples only.
Both antibodies are unconjugated rabbit monoclonal IgGs, with clone IDs R08-4U-7 and R06-7D-5 respectively. They are formulated in 0.01M PBS at pH 7.2 and purified by affinity chromatography.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Optimal working concentrations are not predefined in the product specifications and must be determined by the end user for their specific experimental conditions.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Both antibodies in this pair were raised against a recombinant human IL-25 protein. No additional detail on the immunogen region is provided in the current product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.